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BIOFISICA

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BIOPHYSICS

Codice attività didattica
MFN1199
Docenti
Dott. Alessandra Fiorio Pla (Titolare)
Prof. Luca Munaron
Corso di studio
[f008-c501] LM in Biologia Cellulare e Molecolare (Classe LM-06)
Anno
2° anno
Tipologia
Caratterizzante
Crediti/Valenza
6
SSD attività didattica
BIO/09 - fisiologia
Erogazione
Tradizionale
Lingua
Italiano
Frequenza
Facoltativa
Tipologia esame
Scritto ed orale
Prerequisiti

Conoscenze di base di Fisiologia Generale e Cellulare
Basic knowledge of General and Cellular Physiology

Mutuato da
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Sommario insegnamento

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Obiettivi formativi


Il corso si propone di approfondire alcuni aspetti della fisiologia cellulare.
Ulteriore obiettivo formativo consiste nell’analizzare quantitativamente alcuni approcci tecnici e concettuali ai meccanismi di trasduzione del segnale intracellulare in chiave molecolare e postgenomica, con approfondimenti su temi particolari.
The course aims at focussing the relevant issues of Cell Physiology. An additional objective points to a quantitative analysis of some conceptual and technical approaches to signal transduction mechanisms from a molecular and postgenomic pint of view, with particular emphasis on selected themes.

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Risultati dell'apprendimento attesi


CONOSCENZA E CAPACITÀ DI COMPRENSIONE
conoscenze delle principali acquisizioni della fisiologia cellulare e della moderna interpretazione con gli strumenti della biologia molecolare
CAPACITÀ DI APPLICARE CONOSCENZA E COMPRENSIONE
capacità di utilizzare i concentti acquisiti per l'interpretazione e la comprensione di articoli scientifici
AUTONOMIA DI GIUDIZIO
capacità di valutare criticamente le informazioni, avolte contradditorie, che si ricavanio dalla letteratura
ABILITÀ COMUNICATIVE capacità di presentare in modo sintetico e chiaro i contenuti di un articolo scientifico
CAPACITÀ DI APPRENDIMENTO
capacità di focalizzazione sugli aspetti più rilevanti dell'informazione scientifica
 

KNOWLEDGE AND LEARNING SKILLS. Theoretical and experimental approaches for the quantitative study of cell membranes and intracellular signaling.

USE OF KNOWLEDGE AND LEARNING SKILLS. At the end of the course, the student is expected to be able to:
-  use multiple experimental tools and results to solve a biological problem in cell physiology 

-  discuss the strenght and limitations of the results published on a research paper
-  communicate such findings using appropriate and clear biological language

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Programma

Cell membranes: lipid rafts, caveolae and signalplexes. Membrane permeability. A modern classification of ion channels, transporters and pumps. Functional roles. Ion fluxes: from Fick to Nernst-Planck. Ion channels: classification, structural and functional properties.  How to study ion fluxes through plasmamembrane and intracellular membranes? Electrophysiology: history and techniques. Patch clamp. Amplitude and frequency coding. Postsynaptic and receptor potentials. Action potential and excitability.  

Voltage-gated channels in non excitable cells: role in cell proliferation and tumor progression. Ion channels as proteins: functional role independent from ion fluxes. Role in cell volume regulation. Examples of non voltage-gated channels: TRP channels and PIEZO channels. Spatiotemporal dinamycs of intracellular diffusible second messengers: Ca2+, gasotransmitters, cAMP. Cellular bioimaging using fluorescent probes: FRET, FRAP, TIRF. Optogenetics.

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Modalità di insegnamento

Due to the serious health emergency in progress and in compliance with the measures to contain the spread of Coronavirus infection, by way of derogation from what previously reported in this sheet, the teaching will be provided in alternative teaching methods (scenario 5-integrated solution) using the Moodle platform through the E-Learning DBios website and with telematic meetings on the WebEx platform. Instructions for using the platform are available and accessible on the UniTo website.
The measures introduced are applied on a temporary basis until the end of the emergency situation related to COVID 19.

In particular, the teaching will be provided in the form of: 

1. Lessons and discussion on the program topics live online via webex platform.

2. Recordings of webex lessons uploaded to the Moodle platform.

3. Video-recorded lessons covering the entire program uploaded to the Moodle platform.

 

Lessons and supplementary activities

1. selected readings provided on the Moodle platform;

2. online exercises for self-evaluation provided on the Moodle platform;

3. external in-depth videos on specific topics uploaded to the Moodle platform.

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Modalità di verifica dell'apprendimento

The course grade is determined on the basis of a written examination. It may be integrated by a brief oral discussion.

The examination (2 hours) tests the student's ability to do the following:

- Present briefly the main ideas, concepts and results developed in the course, also explaining the meaning and scope of the experimental approaches behind the validity of the results .


- Use effectively the concepts and the result to answer questions and solve theoretical and experimental problems .

The above is accomplished by asking the student to answer 2-3 open questions and 10 closed questions (multiple choise, short answer and fill in the blanks)
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Testi consigliati e bibliografia

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Lessons, selected papers and websites are available on Moodle.

For some topics selected textbooks available at DBIOS library.



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Orario lezioniV

Nota: Consultare la tabella degli orari pubblicata sull'apposita pagina

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